Epsilon Haemoglobin Specific Antibodies with Applications in Noninvasive Prenatal Diagnosis

Figure 2

Staining of enriched maternal blood sample taken as post-CVS. First enrichment performed by CD-71 directed MACS. Subsequently cells were mounted on glass slides and permeabilized with methanol followed by immunostaining with FITC conjugated DAb1 (30 𝜇g/mL). Subsequently FISH was performed to karyotype the cells. Embryonic nucleated red blood cells (primitive erythroblasts) identified with DAb1, directly conjugated with FITC (green) is shown. Subsequently, FISH was performed against both the X- (blue) and Y-chromosomes (red), confirming the origin of the immunostained cells. X-chromosomes of nonimmunostained nucleated maternal cells (seen as blue dots), are located around the identified foetal erythroblasts.