Research Article

Concentration Polarization of High-Density Lipoprotein and Its Relation with Shear Stress in an In Vitro Model

Table 3

Experimental variables in five locations of model where samples were taken and analyzed for low-speed and high-speed flow.

Location 1Location 2Location 3Location 4Location 5P-value

Shear stress (dyne/cm2 Low-speed19.2115.374.482.970.69
High-speed58.3346.6123.2913.348.96

for HDL (mmol/L Low-speed0.238 (0.237 0.250)0.246 (0.237 0.254)0.27 (0.271 0.284)0.31 (0.292 0.312)0.35 (0.342 0.364)<.001
High-speed0.244 (0.236 0.247)0.248 (0.234 0.250)0.258 (0.234 0.262)0.258 (0.241 0.260)0.26 (0.252 0.271)<.01
P-value .69<.01<.01<.01

ratio  for HD Low-speed0.992 (0.988 1.042)1.025 (0.988 1.058)1.13 (1.129 1.183)1.292 (1.217 1.300)1.46 (1.425 1.517)<.001
High-speed1.017 (0.983 1.029)1.033 (0.975 1.042)1.075 (0.975 1.092)1.075 (1.004 1.083)1.117 (0.050 1.129).049
P-value .69<.01<.01<.01

: concentration at the surface, : ratio of the concentration at the surface ( ) to the concentration in the bulk circulation ( ). Continuous variables were presented as median (range) except for shear stress. Shear stress was constant. Kruskall-Wallis test was used to test the differences among locations, P < .05 was the level of statistical significance, and Mann-Whitney U test with adjusted α was used to test the differences between each two locations (adjusted = 0.0083). Mann-Whitney U test was used to test the difference between speeds. P < .05 was the level of statistical significance. P < .0083 versus location 2. P < .0083 versus location 3. P < .0083 versus location 4.