Rottlerin Inhibits ROS Formation and Prevents NF𝜅B Activation in MCF-7 and HT-29 Cells

Figure 5

Rottlerin prevents both basal and induced intracellular production of H₂O₂. MCF-7 cells were pretreated with 5 and 20 𝜇M Rottlerin for 1 hr and loaded by H₂O₂-specific fluorescent probe DHR-123. Cells were treated with menadione (100 𝜇M) and hydrogen peroxide (100 𝜇M) for 30 min and analyzed using flow cytometry.