Virus-Specific Read-Through Codon Preference Affects Infectivity of Chimeric Cucumber Green Mottle Mosaic Viruses Displaying a Dengue Virus Epitope
Figure 2
A representative gel image of pCG+FSRTRE-derived transcripts without DNase I
treatment electrophoresed after denaturation showing the expected transcript
size (6.5 kb). The band above the transcript band is the DNA template. Lane M:
RNA marker (Invitrogen, Calif, USA);
1, 2, 3: produced transcripts; M1: 1 kb DNA ladder (Promega, Wis, USA); N: pCGT7X, ~9.0 kb; D: DNA template, ~6.5 kb. There were traces of DNA template detected in transcripts
without DNase I treatment.