50Hz Extremely Low Frequency Electromagnetic Fields Enhance Protein Carbonyl Groups Content in Cancer Cells: Effects on Proteasomal Systems
Figure 5
Proteasomal activities measured in cell lysates upon exposure to ELF-EMFs. Caco cells were treated or not with 0.1 M TPA for 24, 48, and 72 hours; they were exposed to ELF-EMFs while the control dishes were placed under the same conditions in a solenoid with no field. After harvesting and lysing the cells, ChT-L (suc-Leu-Leu-Val-Tyr-AMC), T-L (Z-Leu-Ser-Thr-Arg-AMC) PGPH (Z-Leu-Leu-Glu-AMC), and BrAAP (Z-Gly-Pro-Ala-Leu-Ala-pAB) activities were executed in the cell extracts. Data are expressed as fluorescence arbitrary units subtracted of the values of control assays in the presence of specific inhibitors. Data are mean ± SE of six separate experiments. Asterisks represent statistically significant differences from the respective control, not exposed, cultures: .
