VHL Frameshift Mutation as Target of Nonsense-Mediated mRNA Decay in Drosophila melanogaster and Human HEK293 Cell Line
Figure 4
Upf1, Adh, and Vhl expression in Drosophila testes and ovaries. (a) Northern blot analysis of poly (A+) RNAs isolated from wild type adult flies. Lane 1: poly(A+) RNA from ovaries of adult females; lane 2: poly(A+) RNA from testes of adult males. Arrowheads on the left mark the position of the detected mRNAs. (b) RT-PCR analysis of Adh transcripts in wild type adult flies. Lane 1: total RNA from ovaries of adult females; lane 2: total RNA from testes of adult males; lane 3: negative control. (c) RT-PCR analysis of Vhl transcripts in wild type adult flies. Lane 1: total RNA from testes of adult males; lane 2: total RNA from ovaries of adult females; lane 3: negative control.
