BioMed Research International / 2009 / Article / Fig 2

Methodology Report

Improved Coinfection with Amphotropic Pseudotyped Retroviral Vectors

Figure 2

Visualization of coinfection using green and red fluorescent proteins. CRL-2703 human fibroblasts were infected with a 1 : 1 mixture of amphotropic retroviral vectors encoding EGFP and dsRed2. Vectors were used unconcentrated (maximal volume for a well of a 6-well plate: total 7 ml); the same with spinoculation at 1200 g, , 2 hours; concentrated by flocculation with Polybrene from 16 sets of Plat-A cell plates; or combined concentration with Polybrene and spinoculation. Cells were photographed 4 days after infection. Green and red images of representative fields of cells (left and middle) were merged (right) to show coinfected cells (yellow).
901079.fig.002a
(a)
901079.fig.002b
(b)
901079.fig.002c
(c)
901079.fig.002d
(d)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.