Improved Coinfection with Amphotropic Pseudotyped Retroviral Vectors
Visualization of coinfection using green and red fluorescent proteins. CRL-2703 human fibroblasts were infected with a 1 : 1 mixture of amphotropic retroviral vectors encoding EGFP and dsRed2. Vectors were used unconcentrated (maximal volume for a well of a 6-well plate: total 7 ml); the same with spinoculation at 1200 g, , 2 hours; concentrated by flocculation with Polybrene from 16 sets of Plat-A cell plates; or combined concentration with Polybrene and spinoculation. Cells were photographed 4 days after infection. Green and red images of representative fields of cells (left and middle) were merged (right) to show coinfected cells (yellow).
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