BioMed Research International / 2009 / Article / Tab 1

Research Article

Biochemical Characterization of Bovine Brain Myristoyl-CoA:Protein N-Myristoyltransferase Type 2

Table 1

Peptide substrate specificity of the recombinant bovine brain NMT2. The recombinant bovine brain NMT2 assay was measured in the presence of varying concentrations of various peptide substrates (0–1000 μM). The reaction was carried out as described in Section 2. The data shown represent the mean of three replicates. The human NMT1 values were adopted from [28]. Peptides derived from known mammalian N-Myristoyl proteins.

Peptide sequenceKmVmax
Bovine NMT2Human NMT1Bovine NMT2Human NMT1

GSSKSKPK 5.34488120
GNAAAAKKR 441154895
GNASSIKK 8.51367266.8

Peptide derived from NH2-terminal sequence of pp6 . Peptide derived from NH2-terminal sequence of cAMP-dependent protein kinase. Peptide derived from NH2-terminal sequence of M2 gene segment of reovirus type 3.

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