Interfacing Sca-1^pos Mesenchymal Stem Cells with Biocompatible Scaffolds with Different Chemical Composition and Geometry

Figure 3

mTERT protects Sca- 1^pos MSC from oxidative stress induced by high concentrations of 𝐻₂𝑂₂. (a) mTERT-MSC show lower susceptibility to apoptosis induced by high H₂O₂ concentrations. DNA damage measured as the mean Comet %Tail DNA of control MSC and mTERT-MSC cells treated with high H₂O₂ concentrations. Untreated cells were used as negative controls (no damage). The results obtained for the enzyme treatment (hOGG1) were normalized by subtracting the level of %Tail DNA observed with the enzyme-buffer only. The number of cells scored for each sample was 50 and the data are a mean of two experiments. Error bars denote S.E. ^∗𝑃<.005, MSC versus mTERT-MSC.

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