Purification and Characterization of a Lectin from Phaseolus vulgaris cv. (Anasazi Beans)
Figure 1
(a) Fractionation of the crude extract of Anasazi
beans on an Affi-gel blue gel column equilibrated with the binding buffer (10 mM Tris-HCl, pH 7.3). The
column was washed initially with the binding buffer to remove B1 and then
eluted with 1000 mM NaCl in 10 mM Tris-HCl buffer, (pH
7.3) to desorb B2. (b) Superdex 200 column chromatography.
Buffer 20 mM Tris-HCl buffer, (pH
7.3), flow rate: 0.5 mL/min, fraction size: 1.0 mL. Only the major peak (SU1)
exhibited hemagglutinating activity. mAU = milli-absorbance units.
