Generation and Characterization of Novel Human IRAS Monoclonal Antibodies
Production of immunogens for human IRAS. (a) Schematic representation of IRAS fusion used for mAb production. The IRAS N-terminal of the PX domain, and the Arg 10 to Glu 120 were expressed as the NusA fusion protein. LRR represents the leucine-rich repeats and CC represents the coiled-coiled region. Numbers represent amino acid positions. (b) Purification and western blot analysis of NusA-IRA fusion proteins. SDS-PAGE analysis after filtration through the N-NTA column (left column) were then immunoblotted with the anti-His mAb (right column). Sizes (kDa) of molecular mass marker were indicated on the left side.
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