BioMed Research International / 2009 / Article / Fig 2

Research Article

Generation and Characterization of Novel Human IRAS Monoclonal Antibodies

Figure 2

Verification of the specificity of the IRAS mAbs. (a) Identification by western blot using anti-His mAb or the mAbs DA041, DD015, BE073, BA022, and AH021 in NusA and NusA-IRA recombinant proteins. (b) The whole cell extracts derived from HEK293 cells were transfected with pEGFPC1-IRAS and the control vector pEGFPC1 plasmid, respectively. Cell lysates were prepared 48 hours after transfection and were separated by SDS-PAGE. Proteins transferred to PVDF membranes were probed with anti-GFP antibody or the mAbs DA041, DD015, BE073, BA022, and AH021. Sizes (kDa) of molecular mass marker were indicated on the left side.
973754.fig.002a
(a)
973754.fig.002b
(b)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.