Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a
Cre-Expression Vector
Figure 1
(a) Scheme for Cre-loxP-mediated recombination using pCRTEIL and pCETRIZ as reporter
transgenes. Before recombination, the floxed HcRed1/CAT hybrid sequence is
expressed under control of the CAG promoter in cells carrying pCRTEIL, while
the EGFP and luc cDNAs are silent. Similarly, the floxed EGFP/CAT hybrid
sequence is expressed in cells carrying pCETRIZ, but the HcRed1 cDNA and lacZ
genes are silent. Cre-mediated recombination results in deletion of the floxed
sequence, and expression of the EGFP and luc cDNAs in pCRTEIL-carrying cells or
expression of HcRed1 and lacZ in cells carrying pCETRIZ. (b) Plasmids (pCE-29, pCL,
and pCAG/NCre) are used for expression of EGFP, luc, and NCre, respectively.
All plasmids have a pBluescript SK(-) backbone. Abbreviations are CAG:
cytomegalovirus enhancer + chicken -actin promoter; CAP site: transcription start site; CAT: chloramphenicol
acetyltransferase gene; EGFP: enhanced green fluorescent protein cDNA; IRES:
internal ribosomal entry site; Luc: firefly luciferase cDNA; NCre: nuclear
location signal + Cre gene; pA: poly(A) sites.
