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Journal of Biomedicine and Biotechnology
Volume 2009 (2009), Article ID 985140, 9 pages
http://dx.doi.org/10.1155/2009/985140
Research Article

Development of CRTEIL and CETRIZ, Cre-loxP-Based Systems, Which Allow Change of Expression of Red to Green or Green to Red Fluorescence upon Transfection with a Cre-Expression Vector

1Division of Basic Molecular Science and Molecular Medicine, School of Medicine, Tokai University, Bohseidai, Isehara, Kanagawa 259-1193, Japan
2Department of Organ Regeneration, Graduate School of Medicine, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan
3Department of Developmental Biology, National Institute of Agrobiological Sciences, 2 Ikenodai, Tsukuba, Ibaraki, 305-0901, Japan
4Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, 1-21-20 Korimoto, Kagoshima 890-0065, Japan

Received 12 September 2008; Accepted 13 January 2009

Academic Editor: Vladimir Larionov

Copyright © 2009 Masato Ohtsuka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. N. Sternberg and D. Hamilton, “Bacteriophage P1 site-specific recombination. I. Recombination between loxP sites,” Journal of Molecular Biology, vol. 150, no. 4, pp. 467–486, 1981. View at Publisher · View at Google Scholar
  2. R. H. Hoess, M. Ziese, and N. Sternberg, “P1 site-specific recombination: nucleotide sequence of the recombining sites,” Proceedings of the National Academy of Sciences of the United States of America, vol. 79, no. 11, pp. 3398–3402, 1982. View at Publisher · View at Google Scholar
  3. A. Porter, “Controlling your losses: conditional gene silencing in mammals,” Trends in Genetics, vol. 14, no. 2, pp. 73–79, 1998. View at Publisher · View at Google Scholar
  4. K. Araki, M. Araki, J.-I. Miyazaki, and P. Vassalli, “Site-specific recombination of a transgene in fertilized eggs by transient expression of Cre recombinase,” Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 1, pp. 160–164, 1995. View at Publisher · View at Google Scholar
  5. H. Niwa, K. Yamamura, and J. Miyazaki, “Efficient selection for high-expression transfectants with a novel eukaryotic vector,” Gene, vol. 108, no. 2, pp. 193–200, 1991. View at Publisher · View at Google Scholar
  6. M. Sato, Y. Yasuoka, H. Kodama, T. Watanabe, J.-I. Miyazaki, and M. Kimura, “New approach to cell lineage analysis in mammals using the Cre-loxP system,” Molecular Reproduction and Development, vol. 56, no. 1, pp. 34–44, 2000. View at Publisher · View at Google Scholar
  7. M. Sato, T. Watanabe, and M. Kimura, “Embryo transfer via oviductal wall: an alternative method for efficient production of transgenic mice,” Transgenics, vol. 2, no. 4, pp. 383–389, 1999. View at Google Scholar
  8. M. Sato, A. Ishikawa, and M. Kimura, “Direct injection of foreign DNA into mouse testis as a possible in vivo gene transfer system via epididymal spermatozoa,” Molecular Reproduction and Development, vol. 61, no. 1, pp. 49–56, 2002. View at Publisher · View at Google Scholar
  9. M. Sato, “Intraoviductal introduction of plasmid DNA and subsequent electroporation for efficient in vivo gene transfer to murine oviductal epithelium,” Molecular Reproduction and Development, vol. 71, no. 3, pp. 321–330, 2005. View at Publisher · View at Google Scholar
  10. D. G. Kim, H. M. Kang, S. K. Jang, and H.-S. Shin, “Construction of a bifunctional mRNA in the mouse by using the internal ribosomal entry site of the encephalomyocarditis virus,” Molecular and Cellular Biology, vol. 12, no. 8, pp. 3636–3643, 1992. View at Google Scholar
  11. P. Mountford, B. Zevnik, A. Düwel et al., “Dicistronic targeting constructs: reporters and modifiers of mammalian gene expression,” Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 10, pp. 4303–4307, 1994. View at Publisher · View at Google Scholar
  12. M. T. S. Lin, F. Wang, J. Uitto, and K. Yoon, “Differential expression of tissue-specific promoters by gene gun,” British Journal of Dermatology, vol. 144, no. 1, pp. 34–39, 2001. View at Publisher · View at Google Scholar
  13. M. T. S. Lin, L. Pulkkinen, J. Uitto, and K. Yoon, “The gene gun: current applications in cutaneous gene therapy,” International Journal of Dermatology, vol. 39, no. 3, pp. 161–170, 2000. View at Publisher · View at Google Scholar
  14. W. H. Sun, J. K. Burkholder, J. Sun et al., “In vivo cytokine gene transfer by gene gun reduces tumor growth in mice,” Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 7, pp. 2889–2893, 1995. View at Publisher · View at Google Scholar
  15. V. Staniforth, S.-Y. Wang, L.-F. Shyur, and N.-S. Yang, “Shikonins, phytocompounds from Lithospermum erythrorhizon, inhibit the transcriptional activation of human tumor necrosis factor alpha promoter in vivo,” The Journal of Biological Chemistry, vol. 279, no. 7, pp. 5877–5885, 2004. View at Publisher · View at Google Scholar
  16. P. Soriano, “Generalized lacZ expression with the ROSA26 Cre reporter strain,” Nature Genetics, vol. 21, no. 1, pp. 70–71, 1999. View at Publisher · View at Google Scholar
  17. C. G. Lobe, K. E. Koop, W. Kreppner, H. Lomeli, M. Gertsenstein, and A. Nagy, “Z/AP, a double reporter for Cre-mediated recombination,” Developmental Biology, vol. 208, no. 2, pp. 281–292, 1999. View at Publisher · View at Google Scholar
  18. A. Novak, C. Guo, W. Yang, A. Nagy, and C. G. Lobe, “Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision,” Genesis, vol. 28, no. 3-4, pp. 147–155, 2000. View at Publisher · View at Google Scholar
  19. X. Mao, Y. Fujiwara, and S. H. Orkin, “Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice,” Proceedings of the National Academy of Sciences of the United States of America, vol. 96, no. 9, pp. 5037–5042, 1999. View at Publisher · View at Google Scholar
  20. L. St-Onge, P. A. Furth, and P. Gruss, “Temporal control of the Cre recombinase in transgenic mice by a tetracycline responsive promoter,” Nucleic Acids Research, vol. 24, no. 19, pp. 3875–3877, 1996. View at Publisher · View at Google Scholar
  21. K. Akagi, V. Sandig, M. Vooijs et al., “Cre-mediated somatic site-specific recombination in mice,” Nucleic Acids Research, vol. 25, no. 9, pp. 1766–1773, 1997. View at Publisher · View at Google Scholar
  22. F. Schwenk, U. Baron, and K. Rajewsky, “A cre-transgenic mouse strain for the ubiquitous deletion of loxP-flanked gene segments including deletion in germ cells,” Nucleic Acids Research, vol. 23, no. 24, pp. 5080–5081, 1995. View at Publisher · View at Google Scholar
  23. X. Mao, Y. Fujiwara, A. Chapdelaine, H. Yang, and S. H. Orkin, “Activation of EGFP expression by Cre-mediated excision in a new ROSA26 reporter mouse strain,” Blood, vol. 97, no. 1, pp. 324–326, 2001. View at Publisher · View at Google Scholar
  24. T. Motoike, S. Loughna, E. Perens et al., “Universal GFP reporter for the study of vascular development,” Genesis, vol. 28, no. 2, pp. 75–81, 2000. View at Publisher · View at Google Scholar
  25. S. Kawamoto, H. Niwa, F. Tashiro et al., “A novel reporter mouse strain that expresses enhanced green fluorescent protein upon Cre-mediated recombination,” FEBS Letters, vol. 470, no. 3, pp. 263–268, 2000. View at Publisher · View at Google Scholar
  26. K. Vintersten, C. Monetti, M. Gertsenstein et al., “Mouse in red: red fluorescent protein expression in mouse ES cells, embryos, and adult animals,” Genesis, vol. 40, no. 4, pp. 241–246, 2004. View at Publisher · View at Google Scholar
  27. S. Srinivas, T. Watanabe, C.-S. Lin et al., “Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus,” BMC Developmental Biology, vol. 1, article 4, pp. 1–8, 2001. View at Publisher · View at Google Scholar
  28. Y. S. Yang and T. E. Hughes, “Cre stoplight: a red/green fluorescent reporter of Cre recombinase expression in living cells,” BioTechniques, vol. 31, no. 5, pp. 1036–1041, 2001. View at Google Scholar
  29. A. K. Hadjantonakis, S. Macmaster, and A. Nagy, “Embryonic stem cells and mice expressing different GFP variants for multiple non-invasive reporter usage within a single animal,” BMC Biotechnology, vol. 2, article 11, pp. 1–9, 2002. View at Publisher · View at Google Scholar
  30. N. G. Gurskaya, A. F. Fradkov, A. Terskikh et al., “GFP-like chromoproteins as a source of far-red fluorescent proteins,” FEBS Letters, vol. 507, no. 1, pp. 16–20, 2001. View at Publisher · View at Google Scholar
  31. H. Hasuwa, K. Kaseda, T. Einarsdottir, and M. Okabe, “Small interfering RNA and gene silencing in transgenic mice and rats,” FEBS Letters, vol. 532, no. 1-2, pp. 227–230, 2002. View at Publisher · View at Google Scholar
  32. M. D. Muzumdar, B. Tasic, K. Miyamichi, N. Li, and L. Luo, “A global double-fluorescent Cre reporter mouse,” Genesis, vol. 45, no. 9, pp. 593–605, 2007. View at Publisher · View at Google Scholar
  33. S. Nakamura, S. Watanabe, M. Ohtsuka et al., “Cre-loxP system as a versatile tool for conferring increased levels of tissue-specific gene expression from a weak promoter,” Molecular Reproduction and Development, vol. 75, no. 6, pp. 1085–1093, 2008. View at Publisher · View at Google Scholar
  34. M. L. L. Donnelly, G. Luke, A. Mehrotra et al., “Analysis of the aphthovirus 2A/2B polyprotein ‘cleavage’ mechanism indicates not a proteolytic reaction, but a novel translational effect: a putative ribosomal ‘skip’,” Journal of General Virology, vol. 82, no. 5, pp. 1013–1025, 2001. View at Google Scholar
  35. M. L. L. Donnelly, L. E. Hughes, G. Luke et al., “The ‘cleavage’ activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring ‘2A-like’ sequences,” Journal of General Virology, vol. 82, no. 5, pp. 1027–1041, 2001. View at Google Scholar
  36. K. Hasegawa, A. B. Cowan, N. Nakatsuji, and H. Suemori, “Efficient multicistronic expression of a transgene in human embryonic stem cells,” Stem Cells, vol. 25, no. 7, pp. 1707–1712, 2007. View at Publisher · View at Google Scholar