Impact of Lentiviral Vector-Mediated Transduction on the Tightness of a Polarized Model of Airway Epithelium and Effect of Cationic Polymer Polyethylenimine
Figure 7
Effect of PEI/LV vectors and free PEI on occludin localization. Polarized 16HBE41o- cells were incubated with free 1 × 107 PEI molecules (e) or PEI/LV vectors formulated at ratios of 5 × 104 (b), 1 × 106 (c), and 1 × 107 (d) with 50 MOI LV for 24 hours and then evaluated for occludin localization by confocal microscopy. Mock (a) is represented by cells incubated with medium only. Note in green the circumferential localization of occludin between cells, with some intracellular staining. En-face micrographs are shown. Bar: 10 m.
