Efficient Isolation of Cardiac Stem Cells from Brown Adipose
Figure 3
Culture and differentiation of brown adipose-derived cardiac stem cells. Phase-contrast microscopy of differentiated cardiomyocytes-like cells at days 7, 14, and 28. Isolated primary cells were plated into culture dished ( cells /cm2) and observed every day under an inverted phase-contrast microscope at (a)–(c) 1 weeks after spontaneous differentiation, (d)–(f) 2 weeks after spontaneous differentiation, (g)–(i) 4 weeks after spontaneous differentiation. Differentiated cells gradually increased in size with time due to cell proliferation. Early, differentiated cells were rare in plates (day 7), then elongated cells appeared (day 14 and 28) more and more, and they could spontaneously beat like functional cardiomyocytes (bar = 100 m).
