An Improved Harvest and in Vitro Expansion Protocol for Murine Bone Marrow-Derived Mesenchymal Stem Cells
Figure 5
Cell-surface antigens on mMSCs. (a) The yield of ID-mMSCs with modified isolation method is 78.3% higher as compared to the standard isolation methold, /group, *. (b) A representative phenotype of ID-mMSCs from 6 donors at passage 3. Plots show isotype control IgG staining profile (red) versus specific Ab staining profile (white). (c) Contaminated heamatopoietic cells still can be detected in the nonimmunodepleted MSC fraction showing CD14, CD34, and CD45 expression at passage 5, /group, *.
