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Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 121094, 8 pages
Research Article

Construction and Characterization of a Novel Fusion Protein MG7-scFv/SEB against Gastric Cancer

Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan 430022, China

Received 20 September 2009; Revised 4 January 2010; Accepted 13 January 2010

Academic Editor: Xin-yuan Guan

Copyright © 2010 Qiang Tong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Citations to this Article [11 citations]

The following is the list of published articles that have cited the current article.

  • Krishnan Sushma, Mookambeswaran A. Vijayalakshmi, Venkataraman Krishnan, and Padikara Kutty Satheeshkumar, “Cloning, expression, purification and characterization of a single chain variable fragment specific to tumor necrosis factor alpha in Escherichia coli,” Journal of Biotechnology, vol. 156, no. 4, pp. 238–244, 2010. View at Publisher · View at Google Scholar
  • Zuhaida Asra Ahmad, Swee Keong Yeap, Abdul Manaf Ali, Wan Yong Ho, Noorjahan Banu Mohamed Alitheen, and Muhajir Hamid, “scFv Antibody: Principles and Clinical Application,” Clinical and Developmental Immunology, vol. 2012, pp. 1–15, 2012. View at Publisher · View at Google Scholar
  • Xiaoming Lu, Chao Cheng, Guobin Wang, Xiaogang Shu, Jingwei Ma, and Qiang Tong, “Synergistic enhancement of cancer therapy using a combination of fusion protein MG7-scFv/SEB and tumor necrosis factor alpha,” Protein and Peptide Letters, vol. 20, no. 4, pp. 467–472, 2013. View at Publisher · View at Google Scholar
  • Philippe Monnier, Robin Vigouroux, and Nardos Tassew, “In Vivo Applications of Single Chain Fv (Variable Domain) (scFv) Fragments,” Antibodies, vol. 2, no. 2, pp. 193–208, 2013. View at Publisher · View at Google Scholar
  • C-M Cheng, F M Chen, Y-L Lu, S-C Tzou, J-Y Wang, C-H Kao, K-W Liao, T-C Cheng, C-H Chuang, B-M Chen, S Roffler, and T-L Cheng, “Expression of β-glucuronidase on the surface of bacteria enhances activation of glucuronide prodrugs,” Cancer Gene Therapy, vol. 20, no. 5, pp. 276–281, 2013. View at Publisher · View at Google Scholar
  • Rongzhi Wang, Shuangshuang Xiang, Youjun Feng, Swaminath Srinivas, Yonghui Zhang, Mingshen Lin, and Shihua Wang, “Engineering production of functional scFv antibody in E. coli by co-expressing the molecule chaperone Skp,” Frontiers in Cellular and Infection Microbiology, vol. 3, 2013. View at Publisher · View at Google Scholar
  • Z. Li, Y.Q. Li, X.L. Zuo, C.Q. Li, C.J. Zhou, J. Liu, M. Goetz, R. Kiesslich, K.C. Wu, and D.M. Fan, “In vivo molecular imaging of gastric cancer by targeting MG7 antigen with confocal laser endomicroscopy,” Endoscopy, vol. 45, no. 2, pp. 79–85, 2013. View at Publisher · View at Google Scholar
  • Xiao-Dong Wang, Ning-Ning Gao, Yu-Wen Diao, Yu Liu, Dong Gao, Wang Li, Yan-Yan Wan, Jing-Jing Zhong, and Guang-Yi Jin, “Conjugation of toll-like receptor-7 agonist to gastric cancer antigen MG7-Ag exerts antitumor effects,” World Journal Of Gastroenterology, vol. 21, no. 26, pp. 8052–8060, 2015. View at Publisher · View at Google Scholar
  • Forough Yousefi, Seyed Davar Siadat, Alireza Azizi Saraji, Saeed Hesaraki, Mohammad Mehdi Aslani, Seyed Fazlollah Mousavi, and Abbas Ali Imani Fooladi, “Tagging staphylococcal enterotoxin B (SEB) with TGFaL3 for breast cancer therapy,” Tumor Biology, 2015. View at Publisher · View at Google Scholar
  • Ming-Hua Liang, Shi-Shui Zhou, and Jian-Guo Jiang, “Construction, expression and characterization of a fusion protein HBscFv-IFNγ in Komagatella (Pichia) pastoris X33,” Enzyme and Microbial Technology, vol. 102, pp. 74–81, 2017. View at Publisher · View at Google Scholar
  • Haixia Zhang, Xinglong Wang, Xiangrong Li, Zhongren Ma, and Ruofei Feng, “ Construction, expression, and characterization of a single-chain variable fragment (ScFv) antibody targeting to the encephalomyocarditis virus ,” Journal of Medical Virology, 2018. View at Publisher · View at Google Scholar