Insulin Promotes Survival of Amyloid-Beta Oligomers Neuroblastoma Damaged Cells via Caspase 9 Inhibition and Hsp70 Upregulation
Dose dependence of protective effect of insulin against rA42 oligomers induced cell death. (a) LAN5 neuroblastoma cells were untreated (control) or incubated with insulin 50 or 100 or 200 M of insulin alone or after incubation with 25 or 40 M of rA42. After incubation treated and untreated cells were submitted to viability MTS assay. Cell viability is significantly lower in rA42 25 M , rA42 25 M + insulin 50 M versus control, whereas it is not significantly lower with rA42 25 M + insulin 100 M versus control. Cell viability is significantly lower in rA42 40 M , rA42 40 M + insulin 50 M , whereas it is not significantly lower with A 40 M + insulin 100 M versus control. Percentage of viability is referred to control, the data are the mean SD of three separate experiments. (b) Representative morphological images of LAN5 untreated cells (control), treated with rA42 40 M, rA42 40 M, and insulin 100 M, insulin alone 100 M. Bar 20 m.
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