BioMed Research International / 2010 / Article / Fig 1

Research Article

Insulin Promotes Survival of Amyloid-Beta Oligomers Neuroblastoma Damaged Cells via Caspase 9 Inhibition and Hsp70 Upregulation

Figure 1

Dose dependence of protective effect of insulin against rA 𝛽 42 oligomers induced cell death. (a) LAN5 neuroblastoma cells were untreated (control) or incubated with insulin 50 or 100 or 200  πœ‡ M of insulin alone or after incubation with 25 or 40  πœ‡ M of rA 𝛽 42. After incubation treated and untreated cells were submitted to viability MTS assay. Cell viability is significantly lower in rA 𝛽 42 25  πœ‡ M βˆ— 𝑃 < . 0 1 , rA 𝛽 42 25  πœ‡ M + insulin 50  πœ‡ M βˆ— 𝑃 < . 0 1 versus control, whereas it is not significantly lower with rA 𝛽 42 25  πœ‡ M + insulin 100  πœ‡ M versus control. Cell viability is significantly lower in rA 𝛽 42 40  πœ‡ M βˆ— 𝑃 < . 0 0 3 , rA 𝛽 42 40  πœ‡ M + insulin 50  πœ‡ M βˆ— 𝑃 < . 0 0 3 , whereas it is not significantly lower with A 𝛽 40  πœ‡ M + insulin 100  πœ‡ M versus control. Percentage of viability is referred to control, the data are the mean Β± SD of three separate experiments. (b) Representative morphological images of LAN5 untreated cells (control), treated with rA 𝛽 42 40  πœ‡ M, rA 𝛽 42 40  πœ‡ M, and insulin 100  πœ‡ M, insulin alone 100  πœ‡ M. Bar 20  πœ‡ m.
147835.fig.001a
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147835.fig.001b
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