The Masticatory Contractile Load Induced Expression and Activation of Akt1/PKB𝛼 in Muscle Fibers at the Myotendinous Junction within Muscle-Tendon-Bone Unit

Figure 4

Immunoblot analysis of p-Akt1/PKB𝛼 Ser473 in peripheral muscle fibers and in muscle fibers near to the myotendinous areas. Protein extracts prepared from peripheral masticatory muscle fibers and masticatory muscle fibers at MTJ were analyzed by immunoblotting using an antibody against Akt1/PKB𝛼 phosphorylated at Ser473. The weakly phosphorylation of Akt1/PKB𝛼 at Ser473 in peripheral muscle fibers is significantly increased in muscle fibers near to the MTJ. A characteristic band almost at 60 kDa for p-Akt1/PKB𝛼 Ser473 was detectable with a higher staining intensity in muscle fibers near to the MTJ.