163203.fig.004a
163203.fig.004b
163203.fig.004c
Figure 4: Immunoblot analysis of p-Akt1/PKB 𝛼 Ser473 in peripheral muscle fibers and in muscle fibers near to the myotendinous areas. Protein extracts prepared from peripheral masticatory muscle fibers and masticatory muscle fibers at MTJ were analyzed by immunoblotting using an antibody against Akt1/PKB 𝛼 phosphorylated at Ser473. The weakly phosphorylation of Akt1/PKB 𝛼 at Ser473 in peripheral muscle fibers is significantly increased in muscle fibers near to the MTJ. A characteristic band almost at 60 kDa for p-Akt1/PKB 𝛼 Ser473 was detectable with a higher staining intensity in muscle fibers near to the MTJ.