BioMed Research International / 2010 / Article / Fig 5

Research Article

Bone Marrow Derivation of Interstitial Cells of Cajal in Small Intestine Following Intestinal Injury

Figure 5

Representative immunofluorescent image of GFP and c-Kit double positive ICCs on whole mount preparation of small intestine. GFP protein was stained by FITC-labeled goat antirabbit IgG, and c-Kit was demonstrated by Cy3-conjugated goat anti-rat IgG. Upper panel: C57 mouse was used as negative control, in which there were no GFP signals in the intestine. Lower panel: GFP and c-Kit double positive cells were shown in chimeric intestine. Double positive cells could be found on the whole mount preparation, indicating that ICCs could be derived from BMDCs. Yellow arrow was indicated a double positive cell and white arrow directed to its two polar processes. Bar = 5 0 𝜇 m .

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