Research Article

Upregulation of Endogenous HMOX1 Expression by a Computer-Designed Artificial Transcription Factor

Figure 2

Construction of the ATF vectors. PUC57-AZP and pcDNA3.1-NLS-eGFP-p65-flag were double digested at the KpnI (AflII) and BamHI restriction sites respectively to obtain the AZP and pcDNA3.1-p65-flag (pcDNA3.1-NLS-p65-flag) fragments. The two fragments were then recombined to construct pcDNA3.1-NLS-AZP-p65-flag (pcDNA3.1-AZP-p65-flag) vector.
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