168689.fig.002
Figure 2: Construction of the ATF vectors. PUC57-AZP and pcDNA3.1-NLS-eGFP-p65-flag were double digested at the KpnI (AflII) and BamHI restriction sites respectively to obtain the AZP and pcDNA3.1-p65-flag (pcDNA3.1-NLS-p65-flag) fragments. The two fragments were then recombined to construct pcDNA3.1-NLS-AZP-p65-flag (pcDNA3.1-AZP-p65-flag) vector.