BioMed Research International

Research Article

A Human Recombinant Autoantibody-Based Immunotoxin Specific for the Fetal Acetylcholine Receptor Inhibits Rhabdomyosarcoma Growth In Vitro and in a Murine Transplantation Model

Table 2

Primers for scFv from Fab35/38 in orientation VH-VL
scFv/Fab35 V H 5 ′ SfiI: 5 ′ - AGTCTAACGTTCGGCCCAGCCGGCCTCGGGGGGCGACTTGGTCCAGCCGGGGGGG- 3 ′
scFv/Fab38 V H 5 ′ Sfi I: 5 ′ - AGTCTAACGTTCGGCCCAGCCGGCCTCGGGGGGAGGCGTGGTCCAGCCGGGGGGG- 3 ′
scFv/Fab35/38 V H 3 ′ linker: 5 ′ - GCCACCCGACCCACCACCGCCCGAGCCACCGCCACCTGGAGAGACGGTGACCGTTGTCCCTTG
GCC- 3 ′
scFv/Fab35/38 V L 5 ′ linker: 5 ′ - GGCTCGGGCGGTGGTGGGTCGGGTGGCGGCGGATCAGTGATGACCCAGTCTCCA- 3 ′
scFv/Fab35 V L 3 ′ Not I: 5 ′ - TGCTGCTGCGGCCGCTTTGATCTCCAGCTTGGTCCC- 3 ′
scFv/Fab38 V L 3 ′ Not I: 5 ′ -TGCTGCTGCGGCCGCCGTGATCTCCAGCTTGGTCCC- 3 ′
Primers for scFv from Fab35/38 in orientation V L - V H
scFv/Fab35/38 V L 5 ′ Sfi I: 5 ′ - ATGGCTCAGGGTTCGGCCCAGCCGGCCGTGATGACCCAGTCTCCA- 3 ′
scFv/Fab35 V L 3 ′ linker: 5 ′ - GCCACCCGACCCACCACCGCCCGAGCCACCGCCACCTTTGATCTCCAGCTTGGTCCC- 3 ′
scFv/Fab38 V L 3 ′ linker: 5 ′ - GCCACCCGACCCACCACCGCCCGAGCCACCGCCACCCGTGATCTCCAGCTTGGTCCC- 3 ′
scFv/Fab35 V H 5 ′ linker: 5 ′ -GGCTCGGGCGGTGGTGGGTCGGGTGGCGGCGGATCATCGGGGGGCGACTTGGTCCAGCCGG
GGGGG- 3 ′
scFv/Fab38 V H 5 ′ linker: 5 ′ -GGCTCGGGCGGTGGTGGGTCGGGTGGCGGCGGATCATCGGGGGGAGGCGTGGTCCAGCCGG
GGGGG- 3 ′
scFv/Fab35/38 V H 3 ′ Not I: 5 ′ -TGCTGCTGCGGCCGCTGGAGAGACGGTGACCGTTGTCCCTTGGCC- 3 ′