Mouse Homologue of the Schizophrenia Susceptibility Gene ZNF804A as a Target of Hoxc8
Figure 3
RT-PCR analysis of the Zfp804a gene expression in vitro and in vivo. Total cellular RNA was isolated (a) in vitro with F9 cells transfected with pcDNA3 vector (control) and Hoxc8 overexpression vector and (b) in vivo, from mouse E11.5 embryos. (c) Total cellular RNA was isolated from mouse adult whole brain, cortex, and hippocampus (males, 6 weeks old). (d) A ChIP experiment was performed using mouse adult whole brain, cortex, and hippocampus. The DNA-Hoxc8 complexes were immunoprecipitated by a Hoxc8 monoclonal antibody (lane 2). Input (prior to immunoprecipitation) was used for an internal control (lane 1), and mouse IgG and vehicle were used as a negative control (lane 3-4) and DW (lane 5).
