Aquaporin-6 Expression in the Cochlear Sensory Epithelium Is Downregulated by Salicylates

Figure 1

Effect of aspirin on aquaporin-1, -2, -4, -6, and -7 (AQP1, 2, 4, 6, 7) mRNA expression in the mouse inner ear. Representative semiquantitative RT-PCR with specific primers for AQP1, 2, 4, 6, 7 in control (saline) and aspirin treated mice (see Methods). Reverse transcription was performed in the presence (+) or absence (−) of reverse transcriptase enzyme. AQP mRNA expression was normalized to 𝛽-actin. The 327, 441, 383, 309, 437, and 509 bp bands correspond to the AQP-3, -4, -8 and 𝛽-actin-specific PCR products, respectively. Similar results were obtained from at least four different mouse RNA extracts.