Autocrine, Not Paracrine, Interferon-Gamma Gene Delivery Enhances Ex Vivo Antigen-Specific Cytotoxic T Lymphocyte Stimulation and Killing
Transduction efficiency of AAV delivery into DC and T cells by intracellular staining. Cells were infected as described in Section 2 and analyzed by intracellular staining for IFN-γ. (a) shows the results from DC infection. (b) shows the results from T cell infection. Note that both AAV/IFN-γ transduced DC and T cells demonstrate high levels of IFN-γ expression, but that T cells exhibit a signifiucant basal level of expression.
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