A Multifactorial Mechanism in the Superior Antimalarial Activity of 𝛼-C-GalCer

Figure 6

TCR𝛽 usage does not affect in vivo V𝛼14i NKT cell activation stimulated by 𝛼-GalCer or 𝛼-C-GalCer. Groups of 2 WT C57BL/6 were injected i.p. with 1 𝜇g of 𝛼-GalCer or 𝛼-C-GalCer, or with nothing, and 72 hours later splenocytes and hepatic lymphocytes were isolated and stained for V𝛼14i NKT cells using 𝛼-GalCer-loaded mouse CD1d-IgG₁ dimers, as well as for V𝛽8.1/8.2, V𝛽7, and V𝛽2. V𝛼14i NKT cells were gated, and FACS analyzed for the three different V𝛽 gene segments. The numbers shown represent the percentage of cells expressing the indicated V𝛽 segment. The data shown comes from one of two independent experiments with similar results.