The effects of neutrophil incubation on RPEs cell death. Human RPEs were incubated with neutrophils, C2-ceramide (20 μM; positive control), or medium (negative control) for 3 hours and then stained by Propidium iodide (PI). Cell death was evaluated by flow cytometry using 5000 cells for each experiment. The number of nonviable cells (R2) is indicated for each condition. C2-ceramide induced cell death (7.6%). Cells incubated with neutrophils or medium alone had lower cell death, namely, 1.7% and 2.0% cell death, respectively.
