TY - JOUR A2 - De Groot, Anne S. AU - He, Yongqun AU - Xiang, Zuoshuang AU - Mobley, Harry L. T. PY - 2010 DA - 2010/07/04 TI - Vaxign: The First Web-Based Vaccine Design Program for Reverse Vaccinology and Applications for Vaccine Development SP - 297505 VL - 2010 AB - Vaxign is the first web-based vaccine design system that predicts vaccine targets based on genome sequences using the strategy of reverse vaccinology. Predicted features in the Vaxign pipeline include protein subcellular location, transmembrane helices, adhesin probability, conservation to human and/or mouse proteins, sequence exclusion from genome(s) of nonpathogenic strain(s), and epitope binding to MHC class I and class II. The precomputed Vaxign database contains prediction of vaccine targets for >70 genomes. Vaxign also performs dynamic vaccine target prediction based on input sequences. To demonstrate the utility of this program, the vaccine candidates against uropathogenic Escherichia coli (UPEC) were predicted using Vaxign and compared with various experimental studies. Our results indicate that Vaxign is an accurate and efficient vaccine design program. SN - 2314-6133 UR - https://doi.org/10.1155/2010/297505 DO - 10.1155/2010/297505 JF - Journal of Biomedicine and Biotechnology PB - Hindawi Publishing Corporation KW - ER -