Biochemical Signatures of Doppel Protein in Human Astrocytomas to Support Prediction in Tumor Malignancy
Figure 3
Deglycosylation patterns of healthy human testis and glial tumor Dpl. Testis (microsomal fraction) and astrocytomas (soluble fraction) glycoforms of Dpl were analyzed by chemical deglycosylation with TFMS (a) and by enzymatic deglycosylation with neuraminidase and/or PNGase F (b), followed by immunoblotting using a Dpl-specific polyclonal antibody [10, 11]. U: untreated; T: TFMS treatment; N: neuraminidase digestion; P: PNGase F digestion; C: neuraminidase plus PNGase F digestion.
