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Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 301067, 9 pages
http://dx.doi.org/10.1155/2010/301067
Research Article

Biochemical Signatures of Doppel Protein in Human Astrocytomas to Support Prediction in Tumor Malignancy

1Dipartimento di Biochimica, “A. Castellani”, University of Pavia, Via Taramelli 3/b, 27100 Pavia, Italy
2Dipartimento di Genetica e Microbiologia, “A. Buzzati-Traverso”, University of Pavia, Via Ferrata 1, 27100 Pavia, Italy
3IRCCS Policlinico S. Matteo, Via Ferrata 1, 27100 Pavia, Italy
4IGM-CNR, Via Ferrata 1, 27100 Pavia, Italy
5Dipartimento di Patologia Umana e Oncologia, University of Siena, via Banchi di Sotto 55, 53100 Siena, Italy

Received 10 June 2010; Revised 9 September 2010; Accepted 17 September 2010

Academic Editor: Paul Harrison

Copyright © 2010 Paola Rognoni et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Table: Clinical (Anatomical location, WHO grading, Age and Gender) and Dpl expression data (molecular mass, MW; relative quantity, RQ; topology, C=cytosol, M=microsomal, and CM=both) of astrocytoma specimens. Dpl was undetectable (ND) in samples 43-49.

Supplementary Figure: Assessment of the authenticity of Dpl positive bands. A low-grade (2/II) and two high-grade representative astrocytoma samples (23/IV and 33/IV), with both cytosolic and membrane fractions were ran on the same gel along with a Dpl-positive (testis) and a Dpl-negative (healthy brain) control, and similarly immunoblotted using three different anti-Dpl polyclonal antibodies. (A) Dpl-Val, the antibody currently used in all experiments. (B) rabbit anti-Dpl (FL-176) (Santa-Cruz Biotechnology). (C) goat anti-Dpl (N-20) (Santa-Cruz Biotechnology). M: microsomal fraction; C: cytosolic fraction.

  1. Supplementary Table
  2. Supplementary Figure