TNF Induces Choroid Plexus Epithelial Cell Barrier Alterations by Apoptotic and Nonapoptotic Mechanisms
Figure 5
Caspase inhibition prevents TNF-induced DNA fragmentation. Influences of inhibitors of caspases, NF-κB, and JNK were analyzed in respect to TNF-induced PCPEC DNA fragmentation. PCPECs were stimulated apically with TNF (100 ng/mL) with (b) or without (a) cycloheximide (1 g/mL). Cells were additionally preincubated with zVAD (10 M), CAPE (5 M) or SP600125 (10 M) for 2 hours as indicated. Results of these experiments were measured as an optical density (OD). The data shown are mean SEM of three independent experiments, each performed in duplicate; #P-value of <. 05 compared to treated PCPEC without inhibitor.
