TNF Induces Choroid Plexus Epithelial Cell Barrier Alterations by Apoptotic and Nonapoptotic Mechanisms
Figure 6
TNF strongly activates caspase-3 in PCPEC. Caspase-3 activation (given as change of fluorescence per minute in arbitrary units (FU/min.)) was measured in PCPEC after stimulation with TNF (100 ng/mL) for 24 hours with and without zVAD (10 M), CAPE (5 M) or SP600125 (10 M) preincubation for 2 hours (a), and coincubation with cycloheximide (CHX) (1 g/mL) (b) and unstimulated control. The data shown are mean SEM of three independent experiments (), each performed in duplicates. *P-value of <. 05 compared to unstimulated PCPEC; # P-value of <. 05 compared to TNF CHX stimulated PCPEC without inhibitor.
