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Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 317545, 10 pages
Research Article

Rotavirus-Like Particles: A Novel Nanocarrier for the Gut

1UPMC University Paris 06, UMRS 538 “Trafic membranaire et signalisation dans les cellules épithéliales”, 27 rue de Chaligny, 75012 Paris, France
2INSERM UMRS 538 “Trafic membranaire et signalisation dans les cellules épithéliales”, CHU Saint Antoine, 27 rue de Chaligny, 75012 Paris, France
3Unité INRA d'Ecologie et de Physiologie du Système Digestif, Domaine de Vilvert, Jouy-en-Josas, France
4AP-HP, Hôpital Saint-Antoine, Service de Gastroentérologie et Nutrition, 184 rue du Faubourg Saint Antoine, 75012 Paris, France
5Unité de Virologie et Immunologie Moléculaires, UR 089 INRA, 78352 Jouy-en-Josas, France

Received 19 July 2009; Revised 23 November 2009; Accepted 4 February 2010

Academic Editor: Jianguo Wu

Copyright © 2010 Naima G. Cortes-Perez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The delivery of bioactive molecules directly to damaged tissues represents a technological challenge. We propose here a new system based on virus-like particles (VLP) from rotavirus, with a marked tropism for the gut to deliver bio-active molecules to intestinal cells. For this, nonreplicative VLP nanoparticles were constructed using a baculovirus expression system and used to deliver an exogenous biomolecule, the green fluorescent protein (GFP), into either MA104 cells or intestinal cells from healthy and 2,4,6-trinitrobenzene sulfonic acid (TNBS)-treated mice. Our results show that expression of rotavirus capsid proteins in baculovirus led to the auto assembly of VLP that display similar properties to rotavirus. In vitro experiments showed that VLP were able to enter into MA104 cells and deliver the reporter protein. Intragastric administration of fluorescent VLP in healthy and TNBS-treated mice resulted in the detection of GFP and viral proteins in intestinal samples. Our results demonstrate an efficient entry of non-replicative rotavirus VLP into the epithelial cell line MA104 and provide the first in vivo evidence of the potential of these nanoparticles as a promising safe candidate for drug delivery to intestinal cells.