BioMed Research International / 2010 / Article / Fig 1

Research Article

Effects of Garlic Oil on the Migration of Neutrophil-Like Cell Studied by Using a Chemotactic Gradient Labchip

Figure 1

(a) The illustration of chemotactic gradient Labchip. The downstream middle outlet is used to introduce experimental cells and serves as the cell inlet at the beginning of neutrophil-like cell migration experiments. The scale bar is 0.5 mm long. (b) The schematic diagram illustrating the working principle of chemotaxis gradient via chessboard-shaped pillar structure. The background in green demonstrates a gradient distribution over the gradient generator via florescent medium. The scale bar is 1 mm long. (c), (d) and (e) The intensity pictures of the fluorescence medium at the cell migration region under three different input flow rates. The left two buffer inlets were loaded with 5  πœ‡ M FITC-Dextran and DI water, respectively, under the flow rates of 0.5, 1.5, and 2.5  πœ‡ L/min, separately. The scale bar is 100  πœ‡ m long. (f) The normalized fluorescence intensity profiles across the main channel for five different flow rates: 0.5, 1, 1.5, 2, and 2.5  πœ‡ L/min. (g) The image of neutrophil-like cells migration in the microchannel at the cell migration region with an IL-8 gradient of 0–6.25 nM (from top to bottom). Lines indicate trajectories of neutrophil-like cell migration induced by the chemotaxis gradient under a flow rate of 1.5  πœ‡ L/min (flowing from left to right) in 25 minutes. The scale bar is 30  πœ‡ m long.

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