Development of a FLIPR Assay for the Simultaneous Identification of MrgD Agonists and Antagonists from a Single Screen

Table 1

EC₅₀ values obtained for MrgD agonists. Data represents mean ± standard deviation from 2–6 independent experiments.

Ligand	EC50 in 𝜇M
	Human MrgD	Rat MrgD
Beta-alanine	3.76±1.27	3.77±0.87
GABA	53±26	49.8±1.5
Glycine	>100	>100
Gabapentin	No effect	No effect
Pregabalin	No effect	No effect