Development of a FLIPR Assay for the Simultaneous Identification of MrgD Agonists and Antagonists from a Single Screen

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left" rowspan="2">Agonist</td><td align="center" colspan="2">          EC<sub>50</sub> in <svg height="9.6750002" id="M33" style="vertical-align:-2.29482pt" version="1.1" viewbox="0 0 9.6374998 9.6750002" width="9.6374998" xmlns="http://www.w3.org/2000/svg">
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</svg>M</td></tr><tr><td align="center">Human MrgD</td><td align="center">Rat MrgD</td></tr><tr><td align="center" colspan="3"><hr/></td></tr><tr><td align="left">5,   7 dihydroxytryptamine creatinine sulfate</td><td align="center">5.2</td><td align="center">9.7</td></tr><tr><td align="left">Xanthine amine congener</td><td align="center">&gt;100</td><td align="center">48.3</td></tr><tr><td align="left">(<svg height="9.3249998" id="M34" style="vertical-align:-1.09097pt" version="1.1" viewbox="0 0 10.825 9.3249998" width="10.825" xmlns="http://www.w3.org/2000/svg">
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</svg>) HA 966</td><td align="center">11.0</td><td align="center">13.2</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Compounds exhibiting agonism at the MrgD receptor and not the parental CHO-DUKX cells.

BioMed Research International

tab2

Table 2

Table 2: Development of a FLIPR Assay for the Simultaneous Identification of MrgD Agonists and Antagonists from a Single Screen 