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Journal of Biomedicine and Biotechnology
Volume 2010, Article ID 329646, 7 pages
http://dx.doi.org/10.1155/2010/329646
Research Article

Evaluation of Hepcidin Isoforms in Hemodialysis Patients by a Proteomic Approach Based on SELDI-TOF MS

1Department of Medicine, University of Verona, 37134 Verona, Italy
2Nephrology and Haemodialysis Unit, Division of Nephrology, University of Verona, 37134 Verona, Italy
3Department of Medicine and Public Health, University of Verona, 37134 Verona, Italy

Received 30 July 2009; Revised 6 November 2009; Accepted 10 February 2010

Academic Editor: Kai Tang

Copyright © 2010 Natascia Campostrini et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The hepatic iron regulator hormone hepcidin consists, in its mature form, of 25 amino acids, but two other isoforms, hepcidin-20 and hepcidin-22, have been reported, whose biological meaning remains poorly understood. We evaluated hepcidin isoforms in sera from 57 control and 54 chronic haemodialysis patients using a quantitative proteomic approach based on SELDI-TOF-MS. Patients had elevated serum levels of both hepcidin-25 and hepcidin-20 as compared to controls (geometric means: 7.52 versus 4.69 nM, and 4.06 versus 1.76 nM, resp., for both). The clearance effects of a single dialysis session by different dialysis techniques and membranes were also investigated, showing an average reduction by % for hepcidin-25 and % for hepcidin-20 but only minor differences among the different dialysis modalities. Measurement of hepcidin isoforms through MS-based techniques can be a useful tool for better understanding of their biological role in hemodialysis patients and other clinical conditions.