Improved Killing of Human High-Grade Glioma Cells by Combining Ionizing Radiation with Oncolytic Parvovirus H-1 Infection
Figure 2
Effects of ionizing radiation (IR), parvovirus H-1 (H-1PV) infection, and combination of IR and H-1PV infection on human high-grade glioma cells. Short-term cultures of human gliosarcoma NCH-37 (a), human glioblastoma NCH-82 (b), and human glioblastoma NCH-89 (c) were seeded at 30,000 cells/well, irradiated with 5 Gy, 10 Gy, or 20 Gy, and infected with H-1PV at an MOI of 5 PFU/cell 24 hours post-IR (IRH-1) or cells were infected with H-1PV at an MOI of 5 PFU/cell and irradiated with 10 Gy 24 hours post-infection (H-1IR). Effects on cell survival were compared to single treatment with IR or single treatment with H-1PV. Control cells were mock-infected and transported to the accelerator but not exposed to IR (0 Gy). All experiments were performed in three independent assays. Viability (%) was assessed as the number of living treated cells over the number of living untreated cells three days post (mock-) infection. Error bars represent the respective standard error. Significant differences () between single treatment and combination treatment groups are indicated by brackets.
