Molecular Characterization of Heterologous HIV-1gp120 Gene Expression Disruption in <i>Mycobacterium bovis</i> BCG Host Strain: A Critical Issue for Engineering Mycobacterial Based-Vaccine Vectors

<table>Enzymatic restriction analysis of pMV261::HIV-1gp120 and pJH222::HIV-1gp120 plasmid DNA after BCG transformation. <i>Lane 1<b>, </b></i>pMV261 plasmid DNA without HIV-1 gp120 DNA insert digested with BglII; <i>L</i>ane 2 pMV261 plasmid DNA without HIV-1 gp120 DNA insert from BCG colonies, digested with BglII, <i>Lane 3,</i> pMV261-HIV-1gp120 plasmid DNA digested with SacII; <i>lane 4</i>, pMV261-HIV-1gp120 plasmid DNA from rBCG:HIV-1gp120 colonies, digested with SacII; <i>lane 5,</i> pJH222-HIV-1gp120 plasmid DNA digested with HpaI and <i>lane 6</i>, pJH222-HIV-1gp120 plasmid DNA from rBCG:HIV-1 gp120 colonies, digested with HpaI.  Plasmid DNAs before BCG transformation (pre-BCG) were used as positive controls (lane 1, 3 and 5).</table>

BioMed Research International

fig2

Figure 2

Figure 2: Molecular Characterization of Heterologous HIV-1gp120 Gene Expression Disruption in <i>Mycobacterium bovis</i> BCG Host Strain: A Critical Issue for Engineering Mycobacterial Based-Vaccine Vectors 

Figure 2 | Molecular Characterization of Heterologous HIV-1gp120 Gene Expression Disruption in Mycobacterium bovis BCG Host Strain: A Critical Issue for Engineering Mycobacterial Based-Vaccine Vectors 