TY - JOUR A2 - William, Schwan AU - Sun, Juan AU - Hu, Yanxin AU - Cao, Shanping AU - Zhang, Guozhong AU - Sun, Lun-Quan AU - Wang, Ming PY - 2010 DA - 2010/07/20 TI - Acute, Multiple-Dose Dermal and Genetic Toxicity of Nu-3: A Novel Antimicrobial Agent SP - 362524 VL - 2010 AB - Nu-3 [butyl-phosphate-5-thymidine-3-phosphate-butyl] is a modified nucleotide that has been shown to have antimicrobial activity against a range of bacteria including Pseudomonas aeruginosa. However, data on the toxicological profile of Nu-3 are still lacking. In the present study, the toxicity of Nu-3 was evaluated by the following studies: acute oral toxicity, dermal and mucous membrane irritation, multiple-dose toxicity and genotoxicity in vivo and vitro. The acute oral toxicity test in mice showed that Nu-3 had an LD50 of 2001mg/kg body weight. The irritation tests on rats revealed that Nu-3 was not irritant, with an irritation scoring of 0. The multiple-dose toxicity study in rats showed that Nu-3 did not cause significant changes in histology, selected serum chemistry, and hematological parameters compared to the controls. Rats administrated with multiple-doses of Nu-3 showed no visible toxic symptoms. Both in vitro and in vivo, Nu-3 exhibited no notable genetic toxicity. Overall, the data suggest that Nu-3 is hypotoxic or nontoxic antimicrobial compound that warrants being further developed for treating Pseudomonas aeruginosa infection. SN - 2314-6133 UR - https://doi.org/10.1155/2010/362524 DO - 10.1155/2010/362524 JF - Journal of Biomedicine and Biotechnology PB - Hindawi Publishing Corporation KW - ER -