Endonuclease IV Is the Main Base Excision Repair Enzyme Involved in DNA Damage Induced by UVA Radiation and Stannous Chloride

Figure 8

Alkaline gel electrophoresis of E. coli BW527 treated with SnCl₂ (25 𝜇g/mL), UVA (90 kJ/m²) and pre-illuminated with UVA, followed by SnCl₂ incubation. Lanes: (1) control; (2) SnCl₂; (3) UVA; (4) UVA + SnCl₂; (5) SnCl₂ 30 minutes; (6) UVA 30 minutes; (7) UVA + SnCl₂ 30 minutes; (8) SnCl₂ 60 minutes; (9) UVA 60 minutes; (10) UVA + SnCl₂ 60 minutes; (11) SnCl₂ 90 minutes; (12) UVA 90 minutes; (13) UVA + SnCl₂ 90 minutes.

(a) agarose gel electrophoresis

(b) gel densitometrical analysis