BioMed Research International / 2010 / Article / Fig 7

Research Article

The Collagen V Homotrimer Production Is Unexpectedly Favored over the Heterotrimer in Recombinant Expression Systems

Figure 7

Double immunofluorescence staining was performed using monoclonal antibody against human pro 𝛼 1(V) chain (18G5) (a, d, g, j) and rabbit polyclonal pro 𝛼 2(V) chain (b, e, h, m), and merge (c, f, i, l). (a, b, c) human dermal fibroblasts; (d, e, f) clone 22 cells; (g)–(l) HeLa cells transiently transfected with human pro 𝛼 1(V) and pro 𝛼 2(V) constructs. Double immunofluorescence staining shows distinct intracellular distribution of the pro 𝛼 (V) chains in round (g)–(i) and spreading (j, k, l) HeLa cells. Magnification: x1000.

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