Methodology Report

Effective and Steady Differentiation of a Clonal Derivative of P19CL6 Embryonal Carcinoma Cell Line into Beating Cardiomyocytes

Figure 2

Immunofluorescence staining with anticardiac α-actin antibody in P19CL6 cells differentiated by the basal protocol on day 16 ((a) and (c)), and P19CL6-A1 cells by the developed protocol on day 16 ((b) and (d)). ((a) and (c)) Cardiac α-actin-positive cells, various in shape, form relatively small and round clusters. ((b) and (d)) Cardiac α-actin-positive cells, relatively large and elongated in general, formed mesh-like sheet structures. (e) Quantitative analysis of rates of differentiation into cardiomyocytes. Ratios of α-actin-positive cell areas to total areas of microscopic fields were calculated based on a total of 60 micrographs randomly taken from six independent experiments in each cell type and summarized as mean ± SE. Unpaired 𝑡 -test was carried out to compare the differentiation efficiency between the two cells. 𝑃 < . 0 1 .
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(a)
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(b)
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(c)
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(d)
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(e)