Effective and Steady Differentiation of a Clonal Derivative of P19CL6 Embryonal Carcinoma Cell Line into Beating Cardiomyocytes

Table 1

The conditions of differentiation protocol and differentiation efficiency for P19CL6 and P19CL6-A1 cells.

Cell line	P19CL6	PI9CL6-Al	P19CL6
Differentiation protocol	Basal protocol	Developed protocol	Ohtsu et al. [12]
Medium	𝛼-MEM	DMEM	𝛼-MEM
FBS: concentration	6.5%	7.5%	10%
5-Aza: concentration and incubation time	10 𝜇M	10 𝜇M	10 𝜇M
	24 hours	72 hours	24 hours
DMSO: concentration and incubation time	1%	1%	1%
	≥16 days	≥14 days	∼16 days
Coating	Collagen	Collagen	—
	(Type I-A)	(Type I-A)
𝛼-actin-positive cell area to total field*	7.0%	38.3%	—
Relative intensity of 𝛼-MHC mRNA expression**	1.0	1.77	—

∗, ∗∗ Differentiation efficiency measured by immunofluorescence microscopy (∗) and RT-PCR (∗∗) on day 16.