Research Article

Cell Density Plays a Critical Role in Ex Vivo Expansion of T Cells for Adoptive Immunotherapy

Figure 5

Soluble factors secreted by ATCs at high cell density prevent apoptosis of ATCs at low cell density. ATCs cultured at 1 × 1 0 4 /mL in 75 cm2 flasks were supplemented with conditioned medium from (a) ATCs at high cell density or (b) MIP101 cells in different concentrations for 24 hours. Cell viability and intracellular ROS levels were analyzed by flow cytometry. Similar results were obtained in two independent experiments.
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(a)
386545.fig.005b
(b)