Research Article

Enhancement of Human Prolactin Synthesis by Sodium Butyrate Addition to Serum-Free CHO Cell Culture

Figure 7

(a) and (b) Qualitative and quantitative HPSEC analysis of NaBu-treated and untreated CHO-derived hPRL after SP-Sepharose Fast Flow and (c) after preparative HPSEC purification, comparing the product obtained from tube #28 of nontreated (a) and NaBu-treated cells (b). IR: Internal Reference of periplasmic E. coli-derived hPRL (7.0  𝜇 g). mAbs: milliabsorbance. Tube numbers (#27, #28, and #29) are from SP-Sepharose chromatography. Retention Times ( 𝑡 𝑅 ) are indicated.
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(a)
405872.fig.007b
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(c)