BioMed Research International

Research Article

Enhancement of Human Prolactin Synthesis by Sodium Butyrate Addition to Serum-Free CHO Cell Culture

Table 1

Expression levels and RP-HPLC retention times ( 𝑡 𝑅 ) of hPRL obtained from CHO cells treated with different concentrations of NaBu for 10 days. The 𝑡 𝑅 were compared with those of the Internal Reference Preparation of periplasmic E. coli-derived hPRL ( 𝑡 𝑅 ∶ 2 6 . 0 0 ± 0 . 7 2 minutes).
NaBu (mM)Expression levels of h P R L a ( 𝜇 g/mL) 𝑡 𝑅 a (min)
00.92 ± 0.2225.81 ± 0.52
0.251.30 ± 0.0625.68 ± 0.47
0.51.67 ± 0.0025.90 ± 0.77
12.00 ± 0.0325.77 ± 0.73
21.48 ± 0.0525.66 ± 0.87
41.23 ± 0.1125.68 ± 0.82
Average 𝑡 𝑅 = 25.75 ± 0.09
RSD = 0.4%
a Each value is the mean ± SD of duplicate experiments.