Protein-Protein Interaction Changes in an Archaeal Light-Signal Transduction
Figure 6
(a) and (b) minus ppR infrared difference spectra without (red lines) and with (blue lines) pHtrII in the 1735–1675 region. Spectra are measured at 250 K and pH 7 upon hydration with (a) and (b). One division of the - that axis corresponds to 0.005 absorbance units. (c) and (d) Double difference spectra obtained from the minus ppR spectra (a) and (b), where the spectra without pHtrII were subtracted from those with pHtrII. One division of the axis corresponds to 0.0018 absorbance units. (e) and (f) minus ppR infrared difference spectra for the -labeled ppR without (red lines) and with (blue lines) unlabeled pHtrII. (g) and (h) minus ppR infrared difference spectra for the -labeled ppR with unlabeled wild-type (blue lines) and N74T-mutant (orange lines) pHtrII. One division of the axis corresponds to 0.005 absorbance units. Spectra are measured at 250 K and pH 7 upon hydration with (e, g) and (f, h). The original data appeared in [21].
