Differential Proteome Analysis of the Preeclamptic Placenta Using Optimized Protein Extraction
1D-PAGE comparing solubilization and precipitation methods. (a) One-dimensional separation of proteins in PE placenta solubilized in Hepes buffer before and after centrifugation at 43 000 g. From left to right: Mw Precision standard marker, starting material (30 g), supernatant after centrifugation (30 g), and pellet after centrifugation. The gel was stained with Coomassie Brilliant blue. (b) One-dimensional separation of proteins in PE placenta solubilized in urea/CHAPS solution and (c) Hepes buffer. The lanes are from left to right: MW precision standard markers, starting material, and precipitations with acetone, acidified acetone, ethanol, dichloromethane/methanol, TCA, and TCA followed by ethanol wash. Bands indicated with numbers were identified by MALDI-TOF MS (Table 2).
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